CULTIVO DE PROTOPLASTOS PDF

Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. Apartado postal Email Rosa Espejo: respejo lamolina. Department of Biology. New York, NY

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Isolation and regeneration of Magnaporthe grisea protoplasts. I Depto. Protoplasts are important biological tools in filamentous fungi research.

Fungal protoplasts have been extensively used in experiments with genetic transformation. Protoplastization of Magnaporthe grisea was accomplished with Novozym , however, this enzymatic complex is no commercially available for purchase.

Thus, the efficiency of several other commercial enzymes in M. At the same time, osmotic buffer, digestion time and regeneration rate were also analyzed.

The use of 10 or 15 mg of each enzymatic complex in 3 mL of osmotic buffer was most effective for the protoplasts yields. The best osmotic buffer was MgSO 4 1. The M. However, the highest regeneration frequency Additional key words: Protoplastization, rice blast, Pyricularia grisea. Protoplastos de Magnaporthe grisea Hebert Barr.

Para isolar os protoplastos, inicialmente obteve-se cultura-estoque dos isolados. Todos os fatores foram analisados em dois ensaios independentes, e cada tratamento foi aplicado em quatro unidades experimentais erlenmeyer 25 mL.

Agindo individualmente, Cellulase Onozuka R10 liberou protoplastos apenas no segundo ensaio. Este ensaio e os posteriores foram conduzidos apenas com o isolado I A parede celular de M. Dos protoplastos do isolado P2 amostrados por Tanaka et al. Especificamente para M. Os protoplastos de M. Aguilar, M. Asai, T. Isolation and germination type of protoplasts of spores and hyphae of Pyricularia oryzae. Annals of the Phytopathological Society of Japan , Tokyo, v.

Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea by insertional mutagenesis. Molecular Plant-Microbe Interactions , St. Paul, v. Choi, W. The adenylate cyclase gene MAC 1 of Magnaporthe grisea controls appressorium formation and other aspects of growth and development. The Plant Cell , Rockville, v.

Dias, E. Production and regeneration of Penicillium expansum and Penicillium griseoroseum protoplasts. Fincham, J. Transformation in fungi. Microbiological Reviews , Washington, v. Hamlyn, P. Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology , Atlanta, v. Hashiba, T. Formation and purification of protoplasts from Rhizoctonia solani.

Phytopathology , St. Hosokawa, D. Protoplasts isolation of Pyricularia oryzae. A nnals of the Phytopathological Society of Japan , Tokyo, v. Abstract 8. Kim, B. Mycelial protoplast isolation and regeneration of Lentinus lepideus. Life Sciences , Tucson, v. Lalithakumari, D. Protoplasts - a biotechnological tool for plant pathological studies.

Indian Phytopathology , New Delhi, v. Lima, J. Development of a transformation system for Crinipellis perniciosa , the causal agent of witches' broom in cocoa plants. Current Genetics , Berlin, v. Mitchell, T. The cAMP-dependent protein kinase catalytic subunit is required for appressorium formation and pathogenesis by the rice blast pathogen Magnaporthe grisea. Necas, O. Cell wall synthesis in yeast protoplasts.

Bacteriological Reviews , Washington, v. Ou, S. Rice Diseases. Surrey: Commonwealth Mycological Institute. Peberdy, J. Regeneration of Aspergillus nidulans protoplasts. Journal of General Microbiology , London, v. Presidential address: fungi without coats - protoplasts as tools for mycological research.

Mycological Research , Madri, v. Factors affecting protoplast release in some filamentous fungi. Transactions of the British Mycological Society , London, v. Queiroz, M. Strategies for the transformation of filamentous fungi. Journal of Applied Microbiology , Oxford, v.

Schiestl, R. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Shabana, Y. Preparation and regeneration of mycelial protoplasts of Alternaria eichhorniae. Journal of Phytopathology , Berlin, v. Shi, Z. Enhanced transformation in Magnaporthe grisea by restriction enzyme mediated integration of plasmid DNA.

Sweigard, J. Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis. Talbot, N. Identification and characterization of MPG 1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Tanaka, H. Protoplasts of Pyricularia oryzae P2: preparation and regeneration into hyphal form. Agricultural and Biological Chemistry , Tokyo, v.

Teraoka, T. Giant protoplasts of Pyricularia oryzae Cavara. Valent, B. Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics , Pittsburgh, v. Vazquez, F. Protoplasts formation in Fusarium species. Biotechnology Techniques , London, v. Vega, M. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Services on Demand Journal.

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Isolation and regeneration of Magnaporthe grisea protoplasts. I Depto. Protoplasts are important biological tools in filamentous fungi research. Fungal protoplasts have been extensively used in experiments with genetic transformation. Protoplastization of Magnaporthe grisea was accomplished with Novozym , however, this enzymatic complex is no commercially available for purchase.

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Cultivo de células y protoplastos. [2018]

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