Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. Apartado postal Email Rosa Espejo: respejo lamolina. Department of Biology. New York, NY
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Isolation and regeneration of Magnaporthe grisea protoplasts. I Depto. Protoplasts are important biological tools in filamentous fungi research.
Fungal protoplasts have been extensively used in experiments with genetic transformation. Protoplastization of Magnaporthe grisea was accomplished with Novozym , however, this enzymatic complex is no commercially available for purchase.
Thus, the efficiency of several other commercial enzymes in M. At the same time, osmotic buffer, digestion time and regeneration rate were also analyzed.
The use of 10 or 15 mg of each enzymatic complex in 3 mL of osmotic buffer was most effective for the protoplasts yields. The best osmotic buffer was MgSO 4 1. The M. However, the highest regeneration frequency Additional key words: Protoplastization, rice blast, Pyricularia grisea. Protoplastos de Magnaporthe grisea Hebert Barr.
Para isolar os protoplastos, inicialmente obteve-se cultura-estoque dos isolados. Todos os fatores foram analisados em dois ensaios independentes, e cada tratamento foi aplicado em quatro unidades experimentais erlenmeyer 25 mL.
Agindo individualmente, Cellulase Onozuka R10 liberou protoplastos apenas no segundo ensaio. Este ensaio e os posteriores foram conduzidos apenas com o isolado I A parede celular de M. Dos protoplastos do isolado P2 amostrados por Tanaka et al. Especificamente para M. Os protoplastos de M. Aguilar, M. Asai, T. Isolation and germination type of protoplasts of spores and hyphae of Pyricularia oryzae. Annals of the Phytopathological Society of Japan , Tokyo, v.
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Isolation and regeneration of Magnaporthe grisea protoplasts. I Depto. Protoplasts are important biological tools in filamentous fungi research. Fungal protoplasts have been extensively used in experiments with genetic transformation. Protoplastization of Magnaporthe grisea was accomplished with Novozym , however, this enzymatic complex is no commercially available for purchase.
Cultivo de células y protoplastos.